NCI-H520

Name: NCI-H520
Brand: Procell
SKU: CL-0402
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0402

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CL-0402

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NCI-H520
Synonyms	H520;H-520;NCI-HUT-520;NCIH520
Cellosaurus Accession	CVCL_1566
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	lung cancer cells
Tissue	Lung
Age	Age unspecified
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Centrifugation is recommended during subcultivation to prevent cells from growing in dense aggregates.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The NCI-H520 cell line was established in 1982 by A.F. Gazdar et al. from a squamous cell carcinoma of the lung. NCI-H520 cells express significantly reduced levels of p53 mRNA compared with normal lung tissue, but exhibit no gross structural DNA abnormalities. NCI-H520 cells are positive for keratin and vimentin, and negative for neurofilament triplet. NCI-H520 cells form colonies in soft agar.
Tumorigenic	Yes, in nude mice inoculated subcutaneously with 1×10⁷ cells (Tumors developed within 21 days at 100% frequency (5/5)).
Biosafety Level	BSL-1