NCI-H524

Name: NCI-H524
Brand: Procell
SKU: CL-0403
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0403

Publications(2)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H524
Synonyms	H524;H-524;NCIH524
Cellosaurus Accession	CVCL_1568
Organism	Human
Growth Properties	Suspension
Morphology	Round
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: lymph node
Age	63Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The NCI-H524 cell line was established in 1982 from a metastatic lymph node of a male patient with small cell lung cancer.
Biosafety Level	BSL-1