NCI-H69

Name: NCI-H69
Brand: Procell
SKU: CL-0677
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0677

Publications(8)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H69
Synonyms	NCI-H-69;NCI H69;H69;H-69;NCIH69;NCI-HUT-69;H69/P;NCI-H69C;H69C;H69c
Cellosaurus Accession	CVCL_1579
Organism	Human
Growth Properties	Suspension with clustering
Morphology	Lymphoblast-like
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: pleural effusion
Age	55Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.