NCI-H716 [H716]

Name: NCI-H716 [H716]
Brand: Procell
SKU: CL-0405
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0405

Publications(8)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H716 [H716]
Synonyms	NCI H716;H716;H-716;NCIH716
Cellosaurus Accession	CVCL_1581
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Epithelial-like; suspension with partial aggregation
Disease	colorectal cancer cells
Tissue	Cecum; derived from metastatic site: ascites
Age	33Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The NCI-H716 [H716] cell line was established from cells present in ascites fluid obtained from a patient following treatment with 5-fluorouracil. Unlike other colorectal cancer cell lines, NCI-H716 [H716] cells possess dopa decarboxylase and contain dense-core endocrine-type granules in the cytoplasm. NCI-H716 [H716] cells do not express TAG-72 or CA19–9 antigens, nor do they produce carcinoembryonic antigen (CEA).
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Biosafety Level	BSL-1