NCI-H82 [H82]

Name: NCI-H82 [H82]
Brand: Procell
SKU: CL-0712
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0712

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	NCI-H82 [H82]
Synonyms	NCI-H-82;H82;H-82;NCI H82;NCIH82;H82sclc
Cellosaurus Accession	CVCL_1591
Organism	Human
Growth Properties	Suspension
Morphology	Epithelial-like; suspended in clusters
Disease	lung cancer cells
Tissue	Lung; derived from metastatic site: pleural effusion
Age	41Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	NCI-H82 [H82] is a morphological and biochemical variant of small cell lung cancer (SCLC), as the primary tumor from which this cell line derives does not exhibit classic SCLC pathological features. The cells express two canonical SCLC biomarkers, neuron-specific enolase (NSE) and brain-type creatine kinase isoenzyme (CK-BB), while showing undetectable expression of L-DOPA decarboxylase (DDC) and bombesin. Genetically, NCI-H82 [H82] produces an aberrantly sized 3.7 kb TP53 mRNA and harbors approximately 25-fold c-myc gene amplification, accompanied by a 24-fold elevation of c-myc transcript levels compared with normal cells. Functionally, the cell line expresses functional atrial natriuretic peptide (ANP) receptors, yet ANP treatment exerts no effect on its growth characteristics. Immunostaining confirms positive expression of neurofilaments and vimentin; additionally, NCI-H82 [H82] constitutively expresses mRNAs of v-fes, v-fms, H-ras, K-ras, N-ras, and c-raf-1.
Tumorigenic	Yes; Yes, in nude mice.
Biosafety Level	BSL-1