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NCI-H929

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Cat. No.: CL-0509 Publications(10)
NCI-H929 - 1
  • NCI-H929NCI-H929 - 1
  • NCI-H929NCI-H929 - 2
  • NCI-H929NCI-H929 - 3
  • +2

CL-0509

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction

General information
Cell Name NCI-H929
Synonyms NCI H929;NCIH929;H929;H-929
Cellosaurus Accession CVCL_1600
Organism Human
Growth Properties Suspension
Morphology Lymphoblast-like
Disease myeloma cells
Tissue Bone marrow; derived from metastatic site: pleural effusion
Age 62Y
Sex Female
Instructions 1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium RPMI-1640 [PM150110]+0.05 mM Supplement1+10% Nutrient+1% Supplement2
Incubation Atmosphere Air, 95%; CO₂, 5%
Temperature 37℃
Subcultivation Ratio 5×10⁵-1×10⁶ cells/mL
Medium Renewal 2 to 3 times per week
Subculturing Procedure Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.
Biosafety Level BSL-1

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