CL-0509
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Product Introduction
| Cell Name | NCI-H929 |
| Synonyms | NCI H929;NCIH929;H929;H-929 |
| Cellosaurus Accession | CVCL_1600 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | myeloma cells |
| Tissue | Bone marrow; derived from metastatic site: pleural effusion |
| Age | 62Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+0.05 mM Supplement1+10% Nutrient+1% Supplement2 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Biosafety Level | BSL-1 |
Documents
Publications
Journal: PATHOLOGY & ONCOLOGY RESEARCH (2023) IF: 2.8
DOI: 10.3389/pore.2023.1611083
Product Cited: RPMI 8226 Cells Line, U266 [U266B1] Cells Line, NCI-H929 Cells Line, AMO-1 Cells Line
Journal: MOLECULAR BIOTECHNOLOGY (2022) IF: 2.9
DOI: 10.1007/s12033-022-00618-6
Product Cited: NCI-H929 Cells Line, RPMI 8226 Cells Line, U266 [U266B1] Cells Line, Normal Plasma Cells, RPMI-1640, Fetal Bovine Serum
Journal: Molecular Therapy-Oncolytics (2021) IF: 5.7
DOI: 10.1016/j.omto.2021.07.003
Product Cited: A549/DDP Cells Line, COS-1 Cells Line, K-562 [K562] Cells Line, NCI-H929 Cells Line
Journal: Acta Biochimica Et Biophysica Sinica (2021) IF: 3.5
DOI: 10.1093/abbs/gmab128
Product Cited: RPMI 8226 Cells Line, NCI-H929 Cells Line
Journal: Journal of Orthopaedic Surgery and Research (2021) IF: 2.6
DOI: 10.1186/s13018-021-02819-2
Product Cited: RPMI 8226 Cells Line, NCI-H929 Cells Line, U266 [U266B1] Cells Line, RPMI-1640
