Publications(
ManualPM151223
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Product Introduction
Background
Neurobasal medium is a basic neuron culture medium that does not contain L-glutamine and requires the addition of B-27 neuron Supplement with vitamin A, 50× (product number: PB180637). It is suitable for short-term or long-term maintenance and cultivation of neuronal cells in pre-production and embryonic.
Ingredient Statement
With
• 2200 mg/L NaHCO₃
• 4500 mg/L D-Glucose
• 25 mg/L Sodium Pyruvate
• 2600 mg/L HEPES
• 8.1 mg/L Phenol red
Without
• L-Glutamine
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Neurobasal medium complies with ISO 13485, ISO 9001.
Specifications
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.1-7.5 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8℃ for 12 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Publications
Journal: Frontiers in Pharmacology (2025) IF: 4.8
DOI: 10.3389/fphar.2025.1662389
Product Cited: Neurobasal Medium
Journal: BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2025) IF: 4.1
DOI: 10.3967/bes2025.092
Product Cited: Neurobasal Medium, Low-chloride Medium, DMEM (Low glucose), with P/S Medium
Journal: BRAIN RESEARCH BULLETIN (2025) IF: 3.7
DOI: 10.1016/j.brainresbull.2025.111689
Product Cited: B-27 neuron Supplement, without vitamin A, 50 ×, 200 mM L-Alanyl-L-Glutamine Solution, 100 ×, Neurobasal Medium
Journal: ACS Nano (2024) IF: 17.1
Product Cited: Neurobasal medium
Journal: NEUROSCIENCE (2023) IF: 3.3
DOI: 10.1016/j.neuroscience.2023.10.010
Product Cited: Neurobasal medium
FAQs
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Q:1 Can Neurobasal Medium alone or with B-27 Supplement be frozen to extend the shelf life of the product?
AnswerFreezing the culture medium is not recommended because a precipitate may form upon thawing; the inorganic salts and amino acids in the formulation may come out of solution when the temperature fluctuates, and once formed, these precipitates do not easily return to solution.
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Q:2 Does this Neurobasal medium for neuronal culture contain serum? If not, is a serum substitute included?
AnswerThe Neurobasal medium (catalog number PM151223) is serum-free and requires supplementation with B-27. The complete neuronal culture medium and the specialized formulation are composed of Neurobasal + B-27 + additional supplements, and is also serum-free.
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Q:3 Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?
AnswerNo, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
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Q:4 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:5 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:6 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:7 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:8 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:9 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:10 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
