NK-92MI

Name: NK-92MI
Brand: Procell
SKU: CL-0533
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0533

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CL-0533

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	NK-92MI
Synonyms	NK-92 MI;NK-92 mi;NK92-MI;NK92MI;NK-92 transfected with MFG-Hil2
Cellosaurus Accession	CVCL_3755
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Peripheral blood
Age	50Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	MEMα [PM150422]+0.2 mM Inositol+0.1 mM Supplement1+0.02 mM Folic Acid+12.5% Nutrient2+12.5% Nutrient1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Thawing Procedure	NK-92MI常温细胞收货注意事项<br/>1. 细胞刚收到后细胞先竖立着静置2-4个小时，让细胞沉降到底部。<br/>2. 细胞静置完后把瓶中培养基上面部分培养基小心吸出，留底部细胞和3 mL左右培养基在原瓶。<br/>3. 吸出的细胞离心收集细胞沉淀，离心转速1200转3分钟，或者根据您的离心机实际情况而定。<br/>4. 将得到的细胞沉淀用该细胞对应的完培重悬后放回原瓶继续培养过夜，一个T25最终培养体积是5-6 mL。<br/>5. 我们出厂的培养瓶为不透气瓶盖，一定要拧松到不掉的程度，使细胞透气。<br/>6. 培养瓶横放瓶口拧松透气培养过夜。<br/>7. 第二天视细胞密度和培养基消耗情况进行分瓶，可以不离心。
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The NK-92 cell line is an interleukin-2 (IL-2)-dependent natural killer (NK) cell line derived from peripheral blood mononuclear cells of a 50-year-old Caucasian male with rapidly progressive non-Hodgkin's lymphoma. The NK-92MI cell line is an IL-2-independent NK subline generated from NK-92 cells via retroviral transduction. Parental NK-92 cells were transduced with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector; transduction was stable, presumably due to integration of the vector into the host genome. NK-92 cells are cytotoxic to a wide range of malignant cells; chromium-release assays confirm their ability to kill K562 and Daudi cells. NK-92 cells exhibit the following immunophenotypic characteristics: positive for surface markers CD2, CD7, CD11a, CD28, CD45, and CD54; negative for CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34, and HLA-DR. The parental IL-2-dependent NK-92 cell line and a second IL-2-independent subline, NK-92CI, are available from the ATCC. Both NK-92MI and NK-92CI variants contain and express the hIL-2 cDNA and secrete bioactive IL-2; NK-92MI cells produce higher levels of IL-2 than NK-92CI cells, while parental NK-92 cells do not produce IL-2. A culture submitted to ATCC in September 1998 was found to be contaminated with mycoplasma; the progeny were cured of mycoplasma via 21-day treatment with BM Cyclin. Six weeks post-treatment, mycoplasma testing via Hoechst staining, PCR, and standard culture all yielded negative results.

Biosafety Level

BSL-2