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NOMO-1

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Cat. No.: CL-0847 Publications(1)
NOMO-1 - 1
  • NOMO-1NOMO-1 - 1
  • NOMO-1NOMO-1 - 2
  • NOMO-1NOMO-1 - 3
  • +2

CL-0847

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction

General information
Cell Name NOMO-1
Synonyms Nomo-1;NOMO1
Cellosaurus Accession CVCL_1609
Organism Human
Growth Properties Suspension
Morphology Single round cells, suspended
Tissue Bone marrow
Age 31Y
Sex Female
Instructions 1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere Air, 95%; CO₂, 5%
Temperature 37℃
Subcultivation Ratio 1:2-1:3
Medium Renewal Every 2 to 3 days
Subculturing Procedure Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium General Freezing Medium [PB180436]
Storage Conditions For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.
Background NOMO-1 was established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11) (p22;q23) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration.
Biosafety Level BSL-1

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