CL-0847
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | NOMO-1 |
| Synonyms | Nomo-1;NOMO1 |
| Cellosaurus Accession | CVCL_1609 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Single round cells, suspended |
| Tissue | Bone marrow |
| Age | 31Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | Every 2 to 3 days |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | NOMO-1 was established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11) (p22;q23) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration. |
| Biosafety Level | BSL-1 |
Documents
Publications
Integrated analysis and validation reveal CYTH4 as a potential prognostic biomarker in acute myeloid leukemia
Journal: Oncology Letters (2024) IF: 2.9
Product Cited: NOMO-1 Cells Line, MOLM-13 Cells Line, NB4 Cells Line, BALL-1 Cells Line, NALM-6 Cells Line, SUP-B15 Cells Line
