One-step TUNEL Flow Cytometry Apoptosis Kit (Blue, Procell Fluor Violet 450)
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Cat. No.: P-CA-423
P-CA-423
$ 198.00
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Product Introduction
| Detection Principle | When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry. |
| Detection Method | Fluorometric method;Flow cytometry |
| Sample Type | Cell samples |
| Assay Time | 2.5-3 hours |
| Detection Instrument | Flow Cytometer |
| Dye Type | Procell Fluor Violet 450 |
| Ex/Em | 410/450 |
| Channel Set | Pacific Blue |
| Other Reagents Required | PBS buffer (with 1% BSA) (pH7.2-7.4) |
| Storage | This product can be stored at -5~-20°C for 12 months with shading light. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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