One-step TUNEL In Situ Apoptosis Kit (Red, Procell Fluor 647)
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Cat. No.: P-CA-324
P-CA-324
$ 198.00
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Product Introduction
| Detection Principle | When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180-200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180-200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope. |
| Detection Method | Fluorometric method |
| Sample Type | Paraffin section;frozen section;cell slide |
| Assay Time | 3 hours |
| Detection Instrument | Laser confocal microscope ( or Fluorescence microscope with Cy5 filter set) |
| Dye Type | Procell Fluor 647 |
| Ex/Em | 650/665 |
| Filter Set | Cy5 |
| Other Reagents Required | 4% Paraformaldehyde,0.2% Triton X-100,PBS(PB180327),ddH2O,Mounting solution with anti-fluorescence quencher,Xylene,Ethanol |
| Storage | This product can be stored at -5~-20°C for 12 months with shading light. |
| Expiration Date | 12 months |
| Shipping | Ice bag |
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