OPM2
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Cat. No.: CL-0996
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CL-0996
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | OPM2 |
| Synonyms | OPM-2 |
| Cellosaurus Accession | CVCL_1625 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Single, round to polygonal cells in suspension |
| Tissue | Peripheral blood |
| Age | 56Y |
| Sex | 女性 |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Some dead cells are present shortly after recovery; they can be removed by low-speed centrifugation. 4. This cell line has a slow proliferation rate; the subculture split ratio should not be too high. 5. During culture, as cell density increases, the color of the culture medium changes significantly; timely medium exchange is recommended. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-2×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | OPM2 was established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG lambda) in leukemic phase (relapse, terminal) in 1982; described in the literature to carry t(4;14) leading to IGH-FGFR3 (IGH-MMSET) fusion gene. |
| Biosafety Level | BSL-1 |
MSDS
