PC-3-GFP
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Cat. No.: CL-1009
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CL-1009
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | PC-3-GFP |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Epithelial-like |
| Disease | prostate cancer cells |
| Tissue | Prostate; derived from metastatic site: bone |
| Age | 62Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line has a mixed population of suspension and adherent cells. For subculture, collect both the suspension cells from the culture medium and the digested adherent cells, then centrifuge together to pellet the cells. |
| Complete Medium | Ham's F-12K [PM150910]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | PC-3 was isolated from a bone metastatic lesion of grade IV prostatic adenocarcinoma resected from a 62-year-old Caucasian male patient. The cell line displays low acid phosphatase and 5α-reductase enzymatic activity. For drug screening: Fluorescent gene fragments are integrated into the cellular genome through lentiviral transduction, driving intracellular fluorescent protein expression that can be visualized by fluorescence microscopy. This labeling permits convenient tracking and detection of target cells. Fluorescent protein expression intensity may differ across cells due to variable lentiviral integration efficiency. Antibiotic screening can be applied to enrich populations with robust fluorescence. For routine maintenance, periodic puromycin selection at a final concentration of 4 μg/mL is suggested (2–3 times monthly or at user-defined intervals). An additional single round of puromycin selection is required immediately post-thaw of cryopreserved stocks. Daily puromycin supplementation is not needed during regular cultivation. |
| Biosafety Level | BSL-1 |
MSDS
