PC-3-LUC
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Cat. No.: CL-1175
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CL-1175
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | PC-3-LUC |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | prostate cancer cells |
| Tissue | Prostate; derived from metastatic site: bone |
| Age | 62Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | Ham's F-12K [PM150910]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | PC-3 cells were derived from a bone metastasis of a 62-year-old Caucasian male patient with grade IV prostate adenocarcinoma. These cells exhibit low acid phosphatase activity and low 5α-reductase activity. The PC-3-LUC cell line was generated by transducing parental PC-3 cells with a recombinant lentivirus carrying the luciferase gene, yielding a stable cell line with constitutive luciferase expression. This cell line is suitable for a wide range of in vivo and in vitro experiments requiring cell tracing. The puromycin (Puro) screening concentration for this cell line is 10.0 μg/mL. |
| Antigen Expression | HLA A1, A9 |
| Gene Expression | HLA A1, A9 |
| Biosafety Level | BSL-2 |
MSDS
