PC-3-RFP
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Cat. No.: CL-1010
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CL-1010
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | PC-3-RFP |
| Organism | Human |
| Growth Properties | Semi-adherent and semi-suspension |
| Morphology | Epithelial-like |
| Disease | prostate cancer cells |
| Tissue | Prostate; derived from metastatic site: bone |
| Age | 62Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line has a mixed population of suspension and adherent cells. For subculture, collect both the suspension cells from the culture medium and the digested adherent cells, then centrifuge together to pellet the cells. |
| Complete Medium | Ham's F-12K [PM150910]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:6 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | PC-3 was isolated from a bone metastatic lesion of grade IV prostatic adenocarcinoma resected from a 62-year-old Caucasian male patient. The cell line displays low acid phosphatase and 5α-reductase enzymatic activity. For drug screening: Fluorescent gene fragments are integrated into the cellular genome via lentiviral transduction, enabling the cells to express fluorescent proteins observable under a fluorescence microscope and allowing convenient tracking and detection of labeled cells. Variable fluorescent protein expression may occur due to the characteristics of lentiviral integration. Antibiotic selection can be performed to improve fluorescent expression levels. For routine cell culture, periodic supplemental selection with puromycin at a final concentration of 4 μg/mL is recommended, with a frequency of two to three times per month or as customized. One round of puromycin selection is additionally required after thawing cryopreserved cell stocks. Daily puromycin supplementation is not necessary during routine cultivation. |
| Biosafety Level | BSL-1 |
MSDS
