PSN1
Copy the product info.
Cat. No.: CL-0954
-
-
-
- +2
CL-0954
$ 500.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | PSN1 |
| Synonyms | PSN-1 |
| Cellosaurus Accession | CVCL_1644 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | leukemia cells |
| Tissue | Pancreas |
| Age | Age unspecified |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | PSN1 is a cell line exhibiting epithelial morphology, isolated from the pancreas of a patient with adenocarcinoma. Following transplantation into nude mice, the PSN1 cell line was established from the resulting xenograft. PSN1 cells are unique in that amplification of both c-Myc (50-fold) and activated c-Ki-ras (3–6-fold), together with a GGT→CGT point mutation at codon 12, is present at levels similar to those in the original tumor, and they harbor increased levels of c-myc and c-Ki-ras transcripts. Loss of one TP53 allele and a point mutation from AAG to CAG at codon 132 in the remaining allele were also observed in PSN1. |
| Biosafety Level | BSL-1 |
MSDS
