Ramos [RA 1]

Name: Ramos [RA 1]
Brand: Procell
SKU: CL-0483
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0483

Publications(11)

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	Ramos [RA 1]
Synonyms	RAMOS;Ramos 1;RA 1;RA.1;Ra #1;Ra No.1;Ramos(RA1);Ramos-RA1;Ramos (RA 1);Ramos (RA)
Cellosaurus Accession	CVCL_0597
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Ascites; derived from metastatic site: peritoneum
Age	3Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The Ramos [RA 1] cell line was derived from a 3-year-old Caucasian male/female patient with Burkitt's lymphoma. RAMOS cells are EBV-negative, express IL-4 receptors and low-affinity IgE receptors (CD23), and display approximately 1500 IL-4 binding sites per cell. RAMOS cells secrete IgM and express both membrane-bound and secreted forms of immunoglobulin.
Biosafety Level	BSL-1