Rat Brown Adipocytes

Name: Rat Brown Adipocytes
Brand: Procell
SKU: CP-R244
Price: 720.0 USD
Availability: InStock

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Cat. No.: CP-R244

Manual

CP-R244

$ 720.00

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5×10^5Cells/Vial

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Product Introduction Documents FAQs

Product Introduction

General Information

Cell Name	Rat Brown Adipocytes
Species	Rat
Tissue	Brown adipose tissue
Growth Properties	Adherent
Morphology	Spindle-shaped; polygonal; round
Isolation Method	Rat brown adipocytes isolated from Procell Laboratory are prepared by combined collagenase-trypsin digestion to obtain preadipocytes, followed by induction culture in adipogenic induction medium. Each vial contains approximately 5 × 10⁵ cells.
Identity Markers	Rat brown adipocytes isolated from Procell Laboratory exhibit positive Oil Red O staining, with an adipogenic differentiation rate greater than 60%. In addition, the cells test negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Background	Rat brown adipocytes (Rattus norvegicus) are isolated from brown adipose tissue. They are specialized adipocytes containing numerous small lipid droplets and abundant mitochondria in the cytoplasm, and express uncoupling protein 1. Their core functions include: generating heat through non-shivering thermogenesis to maintain body temperature; consuming excess energy to regulate energy balance; and secreting adipokines to participate in metabolic regulation. Reduced quantity or impaired function of rat brown adipocytes is closely associated with obesity, diabetes, metabolic syndrome and cardiovascular diseases. In vitro-cultured rat brown adipocytes serve as a core cell model for investigating energy metabolism and obesity therapy.

Handling Information

Instructions

1. Check all containers for leakage or breakage.
2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
3. Rat brown adipocytes have low buoyant density, adhere poorly, and tend to float. Coating before seeding is recommended.

Reagent Preparation

The culture period of Rat Brown Adipocytes in vitro is limited; it is recommended to use the dedicated growth medium provided by Procell and follow the correct operation methods for cultivation to ensure the optimal culture state of these cells.

Product Name	Cat
Rat Brown Adipocyte Complete Medium	CM-R244
0.25% Trypsin Solution, with EDTA, dissolved in PBS	PB180225
Poly-L-Lysine Solution, 10 ×	PB180523
Phosphate Buffer (PBS, 1 ×)	PB180327

Incubation Atmosphere

Air，95%；CO₂，5%

Temperature

37℃

Subcultivation Ratio

Non‑subculturable

Medium Renewal

Every 2 to 3 days

Population Doublings

Not recommended for expansion or long-term culture

Coating Conditions

PLL (0.1 mg/mL)

Dissociation Reagent

0.25% trypsin

Freeze Medium

General Freezing Medium [PB180436]

Storage Conditions

For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

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Manual

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FAQs

Q:1 The color of cryopreserved cells varies, with some appearing pink and others yellowish. Does this variation affect their viability after thawing?
Answer
This phenomenon frequently occurs between different cryopreservation batches, and is associated with cell density, cryopreservation solution composition, and pH changes induced by temperature variations. This discoloration occurs only occasionally, and does not necessarily indicate compromised cell viability. We recommend attempting cell resuscitation first to assess their condition.
Q:2 How can I improve the survival rate of cryopreserved cells stored at -80°C?
Answer
When storing cryovials, place them in the innermost compartment of the refrigerator's freezer section; avoid placing them on the door shelves or near the refrigerator opening. Additionally, we recommend using refrigerators that are opened less frequently to minimize temperature fluctuations caused by opening and closing the door.
Q:3 How long can cells be preserved in a -80°C freezer?
Answer
The preservation duration of cells at -80°C depends on the operating frequency of the refrigerator and the temperature sensitivity of the cells themselves; therefore, there is no standardized storage period. It is recommended to transfer the cells into liquid nitrogen for long-term storage as early as possible.
Q:4 For frozen cells transported via dry ice, should they be stored at -80°C or in liquid nitrogen upon receipt?
Answer
It is recommended to immediately transfer the received frozen cells into liquid nitrogen for long-term storage; storage at -80℃ should only be used as a short-term interim solution. This is because at -80℃, cellular metabolism is significantly slowed but not completely halted. In addition, frequent opening and closing of the -80℃ freezer causes temperature fluctuations that reduce cell viability.
Q:5 My cells are growing very slowly. What could be the cause of this?
Answer
Please see some common reasons below, and solutions to the issue: - Growth medium is not correct: Use pre-warmed growth medium as recommended by the supplier. - Serum in the growth medium is of poor quality: Use serum from a different lot. and choose good quality serum to ensure nutrition. - Cells have been passaged too many times: Use healthy, low-passage number cells. - Cells were allowed to grow beyond confluency: Passage mammalian cells when they are in the log-phase before they reach confluency. - Culture is contaminated with mycoplasma: Discard cells, media, and reagents. Obtain new stocks of cells, and use them with fresh media and reagents.
Q:6 What factors can contribute to rapid cell death/culture failure?
Answer
There are a number of events that can contribute to this: 1. Incorrect CO2 levels:Monitor the level of CO2 manually with a Fyrite kit, available from Bacharach. Check if the manual readings concur with the readings displayed on the incubator. If the incubator has a trace readout, check the printout for fluctuations in CO2 level. Check the settings to ensure that CO2 levels are set at appropriate levels for your cell line (usually between 5 and 10%). Check line connections frequently for leaks. Avoid frequent opening and closing of incubator doors. 2. Temperature fluctuations in the incubator:Monitor the temperature of incubator with a good thermometer inside the incubator. 3. Amphotericin B or other preventive antibiotics/antimycotics are present at toxic concentrations:Use at recommended levels. 4. Humidity is incorrect:Check the water level in the water pan. Humidity is vital to appropriate gas exchange for many types of cells and media. 5. Incorrect osmotic pressure in medium:Check osmolality of complete medium. Most mammalian cells can tolerate an osmolality of 260 to 350 mOsm/kg. Additions of reagents such as HEPES and drugs may affect osmolality. 6. Contamination by microorganisms:Bacterial and fungal contaminations are usually easily visible; symptoms of mycoplasma contamination are more subtle, and careful monitoring of culture morphology and regular testing are necessary to detect this type of contamination. 7. Inappropriate medium is being used:Double-check that the medium used is appropriate for your cell type and culture application. For example, ensure that the medium being used for serum-free culture is actually designed for serum-free culture; make sure that appropriate selective drugs are used at appropriate levels; check the expiration dates for the reagents being used; and store medium at appropriate temperatures in the dark.