RKO

Name: RKO
Brand: Procell
SKU: CL-0196
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0196

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CL-0196

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	RKO
Cellosaurus Accession	CVCL_0504
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Large intestine; Colon

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is sensitive to temperature changes. The culture medium must be pre-warmed before use; using cold medium will cause cells to round up and contract. It takes more than 72 hours for cells to fully spread after subculture.
Complete Medium	MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	1-2 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	RKO is a poorly differentiated colon cancer cell line. RKO cells harbor wild-type p53 but lack human thyroid hormone receptor β1 (h-TRβ1). The p53 protein level in RKO cells is higher than that in RKO-E6 cells. RKO is the parental cell line of both RKO-E6 and RKO-AS45-1 cells. RKO cells are tumorigenic in nude mice and form colonies in soft agar.
Tumorigenic	Yes, in nude mice. Yes, in soft agar.
Receptor Expression	urokinase receptor (u-PAR)
Biosafety Level	BSL-1