RKO-E6
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Cat. No.: CL-0426
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CL-0426
$ 460.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | RKO-E6 |
| Synonyms | RKOE6 |
| Cellosaurus Accession | CVCL_3787 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | colorectal cancer cells |
| Tissue | Colon |
| Age | Age unspecified |
| Sex | Sex unspecified |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 1-2 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | RKO-E6 cells are a derivative of the human colon cancer RKO cell line transfected with pCMV-E6 using lipofection. RKO-E6 cells contain the human papillomavirus (HPV) E6 oncogene stably integrated under the control of the cytomegalovirus (CMV) promoter. Together with its parental RKO cell line, RKO-E6 can be used to study alterations in cellular parameters caused by p53 deficiency, as well as p53-mediated transcription and apoptosis. |
| Biosafety Level | BSL-2 [Contains HPV6 and CMV viral sequences] |
MSDS
