SCC-25 [SCC 25; SCC25]

Name: SCC-25 [SCC 25; SCC25]
Brand: Procell
SKU: CL-0569
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0569

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CL-0569

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	SCC-25 [SCC 25; SCC25]
Synonyms	SCC 25;SCC25;hSCC-25
Cellosaurus Accession	CVCL_1682
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	tongue squamous cell carcinoma cells
Tissue	Oral cavity; Tongue
Age	70Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. When cells reach overly high confluency, rinse the monolayer twice with PBS. Prolonged incubation in dissociation solution is not recommended, as it will lead to substantial cell loss. Add complete medium for resuspension only after single-cell suspension is confirmed under microscopy.
Complete Medium	DMEM/F12 [PM150312]+10% Nutrient+1% Supplement2+400 ng/mL Supplement1
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.