SH-SY5Y [SHSY-5Y]

Name: SH-SY5Y [SHSY-5Y]
Brand: Procell
SKU: CL-0208
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0208

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CL-0208

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	SH-SY5Y [SHSY-5Y]
Synonyms	SHSY5Y;SHSY-5Y;SH-Sy5y;SK-SH-SY5Y;SY5Y
Cellosaurus Accession	CVCL_0019
Organism	Human
Growth Properties	Adherent with minimal suspension
Morphology	Epithelial-like
Disease	neuroblastoma cells
Tissue	Brain; derived from metastatic site: bone marrow
Age	4Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is prone to cell aggregation. Mild aggregation or even floating on the second day after subculture is a normal phenomenon. Continue culture for 2–3 days without medium exchange, and cells will adhere normally. 4. When severe aggregation affects cell growth, cells can be dissociated with trypsin to re-disperse them. Minimize manipulation and medium changes after subculture, and aggregation will improve.
Complete Medium	Complete culture medium for SH-SY5Y [SHSY-5Y]
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SH-SY5Y [SHSY-5Y] cells are a subline derived from the SK-N-SH neuroblastoma cell line, which was established in 1970 from a bone marrow metastasis of a neuroblastoma. SH-SY5Y was obtained after three rounds of cloning (SK-N-SH → SH-SY → SH-SY5 → SH-SY5Y). SH-SY5Y cells exhibit moderate dopamine-β-hydroxylase activity, and their saturation density is greater than 1 × 10⁶ cells/cm².
Antigen Expression	Blood Type A; Rh+
Biosafety Level	BSL-1