SK-ES-1
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Cat. No.: CL-0997
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CL-0997
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | SK-ES-1 |
| Synonyms | Sk-Es-1;Sk-ES-1;SK ES 01;SK-ES1;SKES-1;SKES1;SK-ES |
| Cellosaurus Accession | CVCL_0627 |
| Organism | Human |
| Growth Properties | Adherent with clustering |
| Morphology | Epithelial-like |
| Tissue | Bone |
| Age | 18Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. The cells show weak adherent properties and mostly form cell aggregates which easily detach and float into the medium. The cells can proliferate normally under suspension culture conditions. |
| Complete Medium | McCoy's 5A [PM150710]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | SK-ES-1 is a cell line exhibiting mesenchymal morphology, isolated from a bone tumor of an 18-year-old Caucasian male patient with anaplastic osteosarcoma. |
| Tumorigenic | 有；是的，在裸鼠中，形成与尤因肉瘤一致的小细胞恶性肿瘤 |
| Antigen Expression | Blood Type A; Rh+ |
| Biosafety Level | BSL-1 |
MSDS
