SK-N-DZ
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Cat. No.: CL-0850
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CL-0850
$ 420.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | SK-N-DZ |
| Synonyms | SKNDZ |
| Cellosaurus Accession | CVCL_1701 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Neuroblast-like |
| Disease | neuroblastoma cells |
| Tissue | Brain; derived from metastatic site: bone marrow |
| Age | 2Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line exhibits weak adherence, so all manipulations must be carried out gently. Pre-warm the culture medium before medium exchange. A small number of floating cells may appear after centrifugation and recovery; perform medium exchange on the next day. Refresh medium every three days. When cell confluence reaches approximately 80%, subculture at a split ratio of 1:2. Minor floating cells after passaging can be eliminated via medium change. Monitor dissociation under a microscope, stop digestion once cells round up and detach; the recommended dissociation time ranges from 4 to 6 minutes. |
| Complete Medium | DMEM [PM150210]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
| Tumorigenic | Yes; Yes, forms tumors in nude mice. |
| Biosafety Level | BSL-1 |
MSDS
