SK-NEP-1

Name: SK-NEP-1
Brand: Procell
SKU: CL-0442
Price: 420.0 USD
Availability: InStock

Copy the product info.

Cat. No.: CL-0442

Publications(1)

Select a size

1×10^6Cells/Vial×2Vials

Quantity

- +

In stock

Inquire

For order requirements and technical needs, please Contact Us

Product Introduction Documents Publications

General information

Cell Name	SK-NEP-1
Synonyms	SKNEP-1;SKNEP1;SKNEP
Cellosaurus Accession	CVCL_0631
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Round
Tissue	kidney
Age	25Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is primarily adherent and requires 3–5 min of digestion.
Complete Medium	McCoy's 5A [PM150710]+15% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Although suspended cells remain viable, their density is low and they may be aspirated and discarded directly. 2. Rinse the adherent cells with PBS, then add 1–2 mL of 0.25% Trypsin-EDTA to the culture flask. Incubate at 37°C. Once the cells round up and detach from the vessel surface, add 4–6 mL of complete medium to terminate digestion. Gently pipette to generate a single-cell suspension, then harvest cells by centrifugation. 3. Seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SK-NEP-1 cells exhibit distinctive ultrastructural features, including sparse microvilli, junctional complexes, morphologically intact Golgi apparatus, abundant smooth endoplasmic reticulum, and cytoplasmic lipid droplets; viral particles are absent in these cells. Published research confirms SK-NEP-1 is classified as an Ewing sarcoma cell line.
Tumorigenic	Yes, in nude mice; forms tumor with small cells consistent with Wilms' tumor.
Antigen Expression	Blood Type A; Rh+
Biosafety Level	BSL-1