SKNO-1

Name: SKNO-1
Brand: Procell
SKU: CL-0927
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0927

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	SKNO-1
Synonyms	Skno-1;SKNO1
Cellosaurus Accession	CVCL_2196
Organism	Human
Growth Properties	Suspension with multicellular aggregation
Morphology	Lymphoblast-like
Tissue	Bone marrow
Age	22Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+10 ng/mL Supplement1+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	2.9-4.3×10⁵ cells/mL
Medium Renewal	every 2 to 3 days
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SKNO-1 was established in 1990 from the bone marrow of a 22-year-old patient with acute myeloid leukemia (AML M2) in second relapse.
Biosafety Level	BSL-1