SU-DHL-2
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Cat. No.: CL-0745
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CL-0745
$ 420.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | SU-DHL-2 |
| Synonyms | SUDHL2;SUDHL-2;Su-DHL-2;SU-DHL2;SuDHL 2;Stanford University-Diffuse Histiocytic Lymphoma-2;DHL-2 |
| Cellosaurus Accession | CVCL_9550 |
| Organism | Human |
| Growth Properties | Suspension with multicellular aggregation |
| Morphology | Lymphoblast-like |
| Tissue | Lymph node |
| Age | 73Y |
| Sex | Female |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:8 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | SU-DHL-2 is a histiocytic cell line that was isolated in 1974 from the lymph node of a White 73-year-old female with large cell lymphoma. The cells are non-specific esterase and acid phosphatase positive. The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). The cells are surface Ig negative (sIG-). The cells are reported to be E - rosette negative. ATCC confirmed this cell line is positive for the presence of Epstein-Barr viral DNA sequences via PCR. |
| Biosafety Level | BSL-1 |
MSDS
