SU-DHL-6

Name: SU-DHL-6
Brand: Procell
SKU: CL-0746
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0746

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

General information

Cell Name	SU-DHL-6
Synonyms	SUDHL6;SuDHL6;SU-DHL6;SUDHL-6;SuDHL-6;Sudhl-6;SUD6;Stanford University-Diffuse Histiocytic Lymphoma-6;DHL-6;DHL6
Cellosaurus Accession	CVCL_2206
Organism	Human
Growth Properties	Suspension
Morphology	Lymphoblast-like
Disease	lymphoma cells
Tissue	Peritoneal effusion
Age	43Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:4
Medium Renewal	Every 3 to 4 days
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SU-DHL-6 is a B-cell non-Hodgkin’s lymphoma (B-NHL) cell line established from the pleural effusion of a 43-year-old patient. The primary tumor was originally classified as diffuse mixed small and large cell lymphoma. This cell line carries the EZH2 Y641N mutation and is categorized as a germinal center B-cell (GCB-like) lymphoma subtype. Exome and RNA sequencing data of SU-DHL-6 are publicly available (see reference 18187).
Gene Expression	monoclonal cytoplasmic immunoglobulin: IgM, lambda light chain
Biosafety Level	BSL-1