SUP-T1
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Cat. No.: CL-0969
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CL-0969
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | SUP-T1 |
| Synonyms | Sup-T1;SUPT-1;SupT-1;Sup T-1;SUP T-1;SUP T1;Sup T1;SupT1;SUPT1;Tsup-1;VB;Stanford University Pediatric T-cell line 1 |
| Cellosaurus Accession | CVCL_1714 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | lymphoma cells |
| Tissue | Pleural effusion |
| Age | 8Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The SUP-T1 cell line was derived from malignant cells isolated from the malignant pleural effusion of an 8-year-old patient with T-cell lymphoblastic lymphoma. This cell line is a suitable host for transfection. |
| Antigen Expression | Leu-6 (CD1a) +; Leu-4 (CD3) +; Leu-3 (CD4) +; Leu-1 (CD5) +; Leu-9 (CD7) +; Leu-2a (CD8) +; OKT-10 (CD38) + |
| Gene Expression | 免疫球蛋白（细胞质） |
| Biosafety Level | BSL-1 |
MSDS
