SW1116

Name: SW1116
Brand: Procell
SKU: CL-0307
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0307

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CL-0307

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	SW1116
Synonyms	SW-1116;SW 1116
Cellosaurus Accession	CVCL_0544
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Colon
Age	73Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Leibovitz's L-15 medium is recommended for this cell line. CO₂ supplementation is not recommended for Leibovitz's L-15 medium, as this may cause cytotoxicity. 4. If a CO₂-free incubator is not available, DMEM may be used as an alternative to Leibovitz's L-15. When using DMEM medium, 5% CO₂ can be supplied normally. 5. This cell line is resistant to standard trypsinization, and serial digestion serves as the standard workflow. Perform each digestion cycle for approximately 7 min: gently flush the monolayer with Ca²⁺/Mg²⁺-free Dulbecco’s Phosphate-Buffered Saline (DPBS) to collect detached cells, then transfer the cell suspension to a centrifuge tube containing complete growth medium for trypsin neutralization. Add fresh Trypsin-EDTA to the culture dish, incubate for another ~7 min, and repeat the cell collection procedure. Small adherent cell populations typically persist after three digestion cycles and are routinely discarded. Additional digestion cycles may be performed to recover residual cells if experimental time permits. Critically, cell suspensions collected within the first three cycles must be subcultured or cryopreserved promptly to avoid marked reductions in cell viability.
Complete Medium	Leibovitz's L-15 [PM151010]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 100%
Temperature	37℃
Subcultivation Ratio	1:2-1:3
Medium Renewal	2 to 3 times per week
Dissociation Duration	7-25 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	SW1116 cells are negative for CSAp (CSAp⁻) and colon antigen 3. They are positive for keratin by immunoperoxidase staining. Oncogene analysis has shown that SW1116 cells express c-myc, K-ras, H-ras, myb, sis, and fos, whereas expression of N-myc and N-ras was not detected. SW1116 cells also express the tumor-specific nuclear matrix proteins CC-4, CC-5, and CC-6.
Tumorigenic	Yes, in nude mice.
Antigen Expression	Blood Type O; Rh+
Gene Expression	carcinoembryonic antigen (CEA) 2654 ng/10⁶ cells/10 days; keratin
Biosafety Level	BSL-1