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CL-0742
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Product Introduction
| Cell Name | SW1463 |
| Synonyms | SW-1463;SW 1463 |
| Cellosaurus Accession | CVCL_1718 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | colorectal cancer cells |
| Tissue | Large intestine; Rectum |
| Age | 66Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Only Leibovitz's L-15 medium can be used for this cell line. CO₂ supplementation is not permitted for Leibovitz's L-15, as it will cause cytotoxicity. 4. This cell line adheres firmly and has a slow proliferation rate. |
| Complete Medium | Leibovitz's L-15 [PM151010]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 100% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 10-15 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Tumorigenic | Yes, in nude mice. (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Receptor Expression | unknown |
| Antigen Expression | Blood Type A; Rh+ |
| Gene Expression | carcinoembryonic antigen (CEA) 223 ng/10⁶ cells/10 days; keratin |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Discover Oncology (2025) IF: 2.9
DOI: 10.1007/s12672-025-03075-0
Product Cited: Human Astrocytes, MDA-MB-468 Cells Line, Caki-2 Cells Line, THLE-2 Cells Line, RWPE-1 Cells Line, NCI-H226 Cells Line, HuCC-T1 Cells Line, HK-2 Cells Line, ACHN Cells Line, SV-HUC-1 Cells Line, HCT 116 Cells Line, Human Epidermal Melanocytes, A549 Cells Line, MCF 10A Cells Line, KYSE-30 Cells Line, FaDu Cells Line, A-375 Cells Line, Human Intrahepatic Bile Duct Epithelial Cells, UM-UC-3 Cells Line, Hep G2 Cells Line, AGS Cells Line, LNCaP clone FGC Cells Line, U251 Cells Line, SW1463 Cells Line
Journal: EXPERIMENTAL CELL RESEARCH (2023) IF: 3.7
DOI: 10.1016/j.yexcr.2023.113733
Product Cited: SW1463 Cells Line, SW1116 Cells Line
