SW480 [SW-480]

Name: SW480 [SW-480]
Brand: Procell
SKU: CL-0223
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0223

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CL-0223

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	SW480 [SW-480]
Synonyms	SW-480;SW 480;SW480E
Cellosaurus Accession	CVCL_0546
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	colorectal cancer cells
Tissue	Large intestine; Colon
Age	50Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. Leibovitz's L-15 medium is recommended for this cell line. CO₂ supplementation is not recommended for Leibovitz's L-15 medium, as this may cause cytotoxicity.
Complete Medium	Leibovitz's L-15 [PM151010]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 100%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The SW480 [SW-480] cell line was derived from a primary colon adenocarcinoma; the SW620 cell line was established from a lymph node metastasis obtained from the same patient one year later. SW480 cells are negative for CSAp and rectal antibody 3, and positive for keratin as determined by immunoperoxidase staining. The TP53 gene harbors a G→A transition at codon 273, resulting in an Arg→His substitution, and a C→T transition at codon 309, resulting in a Pro→Ser substitution. Cellular p53 protein expression is elevated. The oncogenes c-myc, K-ras, H-ras, N-ras, c-myb, c-sis, and c-fos are expressed, whereas N-myc expression was not examined. SW480 cells do not express cytolysin, a metalloproteinase associated with tumor invasion. SW480 cells have been reported to express GM-CSF. The KRAS oncogene contains a mutation at codon 12, which can serve as a positive control for PCR-based detection of this mutation. When A. Leibovitz submitted the cell line to ATCC in November 1978, it had reached passage 91.
Tumorigenic	Yes. Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells.
Receptor Expression	epidermal growth factor (EGF)
Gene Expression	carcinoembryonic antigen (CEA) 0.7 ng/10⁶ cells/10 days; keratin; transforming growth factor beta, myc+; myb+ ; ras+; fos+; sis+; p53+; abl -; ros -; src -, HLA A2, B8, B17; Blood Type A; Rh+, The cells are positive for keratin by immunoperoxidase staining., The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes.
Biosafety Level	BSL-1