SW900
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Cat. No.: CL-0851
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CL-0851
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | SW900 |
| Synonyms | SW-900; SW 900 |
| Cellosaurus Accession | CVCL_1731 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Tissue | Lung |
| Age | 65Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line has irregular morphology with vacuoles, and a small number of floating cells. 4. This cell line proliferates slowly. 5. Leibovitz's L-15 medium can be used for this cell line. CO₂ supplementation is not permitted for Leibovitz's L-15, as it will cause cytotoxicity. 6. High-density culture is recommended for this cell line. |
| Complete Medium | Advanced DMEM [PM153210]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 100% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The SW900 cell line was established in January 1975 from lung biopsy tissue of a 65-year-old White male patient with pulmonary squamous cell carcinoma. |
| Tumorigenic | Yes; Yes, produces tumors in nude mice consistent with type II broncheolar adenocarcinoma |
| Antigen Expression | Blood type A; Rh+ |
MSDS
