T84

Name: T84
Brand: Procell
SKU: CL-0229
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0229

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CL-0229

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	T84
Synonyms	T-84;T 84
Cellosaurus Accession	CVCL_0555
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Colon; derived from metastatic site: lung
Age	72Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line exhibits highly aggregated growth. Cell aggregation causes partial cell death during culture, resulting in floating cells and cell debris. Meanwhile, this cell line has a fast metabolic rate, requiring timely medium exchange. 4. Floating cells appear during cell culture, particularly noticeable on the second day after subculture. Cells will adhere and grow after medium exchange every 2–3 days.
Complete Medium	DMEM/F12 [PM150312]+5% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2-1:4
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	Freezing Medium (Serum-free & animal origin-free) [PB180438]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice. Throughout the transplantation procedure in nude mice, the cell line maintained the original histological characteristics of the colon carcinoma. After 23 passages in athymic mice, the T84 cell line was established. T84 cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport. T84 cells are positive for keratin by immunoperoxidase staining.
Tumorigenic	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10⁷ cells).
Receptor Expression	neurotransmitter, expressed; peptide hormone, expressed
Gene Expression	carcinoembryonic antigen (CEA), 600ng/mL per 1×10⁶ cells per 10 days; keratin
Biosafety Level	BSL-1