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CL-0628
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Product Introduction
| Cell Name | TCCSUP |
| Synonyms | TCCSuP;TCC-SUP;TCC Sup |
| Cellosaurus Accession | CVCL_1738 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | bladder cancer cells |
| Tissue | Urinary bladder |
| Age | 67Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 1-2 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The TCCSUP line was isolated in 1974 from an anaplastic transitional cell carcinoma (TCC) in the neck of the urinary bladder. |
| Antigen Expression | HLA 2, 3, 7, 12 |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: ONCOGENE (2026) IF: 7.3
DOI: 10.1038/s41388-026-03688-x
Product Cited: TCCSUP Cells Line, 5637 Cells Line, HT-1197 Cells Line, SW1710 Cells Line, T24 Cells Line, SV-HUC-1 Cells Line
Journal: CANCER IMMUNOLOGY IMMUNOTHERAPY (2026) IF: 5.1
DOI: 10.1007/s00262-026-04353-8
Product Cited: Jurkat, Clone E6-1 Cells Line, THP-1 Cells Line, 253J Cells Line, SV-HUC-1 Cells Line, RT4 Cells Line, T24 Cells Line, TCCSUP Cells Line, MB49 Cells Line, J82 Cells Line, 5637 Cells Line, 293T Cells Line
Journal: Theranostics (2025) IF: 12.4
DOI: 10.7150/thno.103175
Product Cited: TCCSUP Cells Line, T24 [T-24] Cells Line
Journal: MAMMALIAN GENOME (2025) IF: 2.7
DOI: 10.1007/s00335-025-10182-4
Product Cited: T24 Cells Line, TCCSUP Cells Line, McCoy's 5A Medium, MEM, with NEAA Medium, SV-HUC-1 Cells Line, Ham's F-12K Medium
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2025) IF: 2.2
DOI: 10.1016/j.bbrc.2025.153036
Product Cited: 5637 Cells Line, TCCSUP Cells Line, RPMI-1640 Medium, Human Bone Marrow-Derived Macrophage Complete Medium, Fetal Bovine Serum, Human Bone Marrow-Derived Macrophages, Penicillin-Streptomycin Solution, 100 ×, HT-1376 Cells Line, SW1710 Cells Line, RT-112 Cells Line, SV-HUC-1 Cells Line
