TF-1

Name: TF-1
Brand: Procell
SKU: CL-0232
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0232

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CL-0232

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	TF-1
Synonyms	TF1;MFD-1
Cellosaurus Accession	CVCL_0559
Organism	Human
Growth Properties	Semi-adherent and semi-suspension
Morphology	Lymphoblast-like
Disease	leukemia cells
Tissue	Bone marrow
Age	35Y
Sex	Male

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+2 ng/mL Supplement1+10% Nutrient+1% Supplement2
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-5×10⁵ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	1. This cell line displays mixed adherent and suspended phenotypes. Suspended cells remain viable. Transfer the single-cell suspension into a centrifuge tube and pellet the cells via centrifugation at 1200 × g (approximately 250 × g). 2. Adherent cells can be dispersed by gentle pipetting to form a homogeneous single-cell suspension. 3. Mix all harvested suspended and adherent cell populations, then seed the cells into fresh culture vessels at the recommended split ratio.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The TF-1 cell line was established in 1987 by T. Kitamura et al. from a heparinized bone marrow aspirate obtained from a 35-year-old Japanese male with severe pancytopenia. TF-1 cells are strictly dependent on IL-3 or GM-CSF for long-term growth and do not respond to IL-5. Various lymphokines and cytokines, including IL-1, IL-4, IL-6, IL-9, IL-11, IL-13, G-CSF/GM-CSF, LIF, and NGF, exert biological effects on TF-1 cells. The cells do not express glycophorin A or carbonic anhydrase I. Their morphological and cytochemical characteristics, together with constitutive globin gene expression, indicate that TF-1 cells are committed to the erythroid lineage.

Biosafety Level

BSL-1