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CL-0233
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Product Introduction
| Cell Name | THP-1 |
| Synonyms | THP1;THP 1;THPI;THP-1(ATCC);Tohoku Hospital Pediatrics-1 |
| Cellosaurus Accession | CVCL_0006 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Monocytic |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 1Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+0.05 mM Supplement1+1% Supplement2 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | THP-1 cells are phagocytic toward latex beads and sensitized erythrocytes and lack surface and cytoplasmic immunoglobulins. THP-1 cells can be induced to undergo monocytic differentiation by phorbol 12-myristate 13-acetate (TPA/PMA). |
| Receptor Expression | complement (C3), expressed;Fc, expressed |
| Antigen Expression | HLA A2, A9, B5, DRw1, DRw2 |
| Gene Expression | lysozyme, HLA A2, A9, B5, DRw1, DRw2 |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Scientific Reports (2024) IF: 3.8
DOI: 10.1038/s41598-024-69389-1
Product Cited: TM4 Cells Line, THP-1 Cells Line, Donor Equine Serum, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Medium
Journal: Scientific Reports (2024) IF: 3.8
DOI: 10.1038/s41598-024-71771-y
Product Cited: THP-1 Cells Line
Journal: Journal of Ovarian Research (2024) IF: 3.8
DOI: 10.1186/s13048-024-01430-3
Product Cited: THP-1 Cells Line, A2780 Cells Line, SK-OV-3 Cells Line
Journal: JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY (2024) IF: 3.7
DOI: 10.1007/s13105-024-01058-3
Product Cited: RAW 264.7 Cells Line, Fetal Bovine Serum, THP-1 Cells Line, Penicillin-Streptomycin Solution, 100 ×, DMEM (High glucose) Medium
Journal: MOLECULAR IMMUNOLOGY (2024) IF: 3.6
DOI: 10.1016/j.molimm.2024.01.002
Product Cited: RAW 264.7 Cells Line, THP-1 Cells Line, H9c2 (2-1) Cells Line, HL-1 Cells Line, Human Aortic Smooth Muscle cells, Human Umbilical Vein Endothelial cells, MEM, DMEM(High glucose), Ham's F-12K, RPMI-1640, Fetal Bovine Serum
