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CL-0233
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Product Introduction
| Cell Name | THP-1 |
| Synonyms | THP1;THP 1;THPI;THP-1(ATCC);Tohoku Hospital Pediatrics-1 |
| Cellosaurus Accession | CVCL_0006 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Monocytic |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 1Y |
| Sex | Male |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+0.05 mM Supplement1+1% Supplement2 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | THP-1 cells are phagocytic toward latex beads and sensitized erythrocytes and lack surface and cytoplasmic immunoglobulins. THP-1 cells can be induced to undergo monocytic differentiation by phorbol 12-myristate 13-acetate (TPA/PMA). |
| Receptor Expression | complement (C3), expressed;Fc, expressed |
| Antigen Expression | HLA A2, A9, B5, DRw1, DRw2 |
| Gene Expression | lysozyme, HLA A2, A9, B5, DRw1, DRw2 |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: Nature Communications (2025) IF: 15.7
DOI: 10.1038/s41467-025-62645-6
Product Cited: 293T Cells Line, THP-1 Cells Line, 293A Cells Line
Journal: Nature Communications (2025) IF: 15.7
DOI: 10.1038/s41467-025-62968-4
Product Cited: THP-1 Cell Complete Medium, THP-1 Cells Line, RAW 264.7 Cells Line
Journal: Nature Communications (2025) IF: 15.7
DOI: 10.1038/s41467-025-63198-4
Product Cited: THP-1 Cells Line, 4T1 Cells Line
Journal: Nature Communications (2025) IF: 15.7
DOI: 10.1038/s41467-025-63348-8
Product Cited: 293T Cells Line, THP-1 Cells Line
Journal: Nature Communications (2025) IF: 15.7
DOI: 10.1038/s41467-025-64181-9
Product Cited: HL-1 Cells Line, Human Peripheral Blood Monocyte Complete Medium, Hep 3B2.1-7 Cells Line, 293T Cells Line, Mouse Brain Microvascular Pericytes, Mouse Brain Microvascular Endothelial Cell Complete Medium, Mouse Peripheral Blood Monocyte Complete Medium, Renca Cells Line, MRC-5 Cells Line, THP-1 Cells Line, SH-SY5Y Cells Line, MEM/F12 Medium, Neuro-2a Cells Line, Mouse Brain Microvascular Pericyte Complete Medium, bEnd.3 Cells Line, Mouse Astrocyte Complete Medium, Mouse Astrocytes, PANC-1 Cells Line, HuH-7 Cells Line, Hepa 1-6 Cells Line, Mouse Brain Microvascular Endothelial Cells, C8-D1A Cells Line
