THP-1-GFP
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Cat. No.: CL-1124
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CL-1124
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | THP-1-GFP |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Monocytic |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 1Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+0.05 mM Supplement1+1% Supplement2 |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | THP-1 cells are phagocytic toward latex beads and sensitized erythrocytes and lack surface and cytoplasmic immunoglobulins. THP-1 cells can be induced to undergo monocytic differentiation by phorbol 12-myristate 13-acetate (TPA/PMA). The THP-1-GFP cell line was constructed from THP-1 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 11.0 μg/mL. |
| Receptor Expression | complement (C3), expressed;Fc, expressed |
| Antigen Expression | HLA A2, A9, B5, DRw1, DRw2 |
| Gene Expression | lysozyme, HLA A2, A9, B5, DRw1, DRw2 |
| Biosafety Level | BSL-1 |
MSDS
