TK6

Name: TK6
Brand: Procell
SKU: CL-0813
Price: 600.0 USD
Availability: InStock

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Cat. No.: CL-0813

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1×10^6Cells/Vial×2Vials

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General information

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	2×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	TK6 is a lymphoblastoid cell line isolated from the spleen of a 5-year-old male with hereditary spherocytosis. These cells are heterozygous at the thymidine kinase (TK) locus and can be used to quantitatively detect forward mutations at the tk locus (resistance to trifluorothymidine).
Biosafety Level	BSL-2