Publications(
CL-1035
$ 500.00
Select a size
1×10^6Cells/Vial×2Vials
Quantity
-
+
In stock
InquireFor order requirements and technical needs, please Contact Us
Product Introduction
General information
| Cell Name | TMD8 |
| Synonyms | TMD-8;Tokyo Medical and Dental university 8 |
| Cellosaurus Accession | CVCL_A442 |
| Organism | Human |
| Growth Properties | Suspension with clustering |
| Morphology | Round; growing in clusters |
| Disease | Diffuse large B-cell lymphoma |
| Tissue | Bone Marrow |
| Age | 62Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | TMD8 was established from the bone marrow of a 62-year-old Japanese male patient with diffuse large B-cell lymphoma. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Nuclear receptor subfamily 2 group F member 2 transcriptionally activates 14-3-3 epsilon to promote diffuse large B-cell lymphoma progression
Journal: CYTOTECHNOLOGY (2026) IF: 1.7
DOI: 10.1007/s10616-026-00965-1
Product Cited: SU-DHL-4 Cells Line, TMD8 Cells Line, DB Cells Line, RPMI-1640 Medium
