TT

Name: TT
Brand: Procell
SKU: CL-0457
Price: 460.0 USD
Availability: InStock

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Cat. No.: CL-0457

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CL-0457

$ 460.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	TT
Synonyms	MTC-TT
Cellosaurus Accession	CVCL_1774
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Disease	other cancer cells
Tissue	Thyroid; Medulla
Age	77Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line has slow proliferation and requires 1–2 weeks to reach satisfactory growth when subcultured at a 1:2 split ratio. It has high serum dependence, and serum supplementation up to 20% is permissible. Given its slow population growth, medium renewal once every three days is sufficient. Frequent manipulations are not recommended, and the split ratio for subcultivation should not be lower than 1:4.
Complete Medium	Ham's F-12K [PM150910]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:2
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The TT cell line was established from a needle biopsy specimen from a 77-year-old female patient with medullary thyroid carcinoma. TT cells constitutively produce high levels of calcitonin and CEA. Immunoreactive calcitonin levels of 3900 pg/million cells and 7700 pg/million cells were measured in the culture medium at 24 and 72 hours after medium change, respectively. After 72 hours, CEA accumulation exceeded 27 ng/million cells.

Biosafety Level

BSL-1