U-2 OS [U2OS]

Name: U-2 OS [U2OS]
Brand: Procell
SKU: CL-0236
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0236

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CL-0236

$ 420.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	U-2 OS [U2OS]
Synonyms	U-2 OS;U-2OS;U-2-OS;U2-OS;U20-S;U20S;2T
Cellosaurus Accession	CVCL_0042
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Bone
Age	15Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	McCoy's 5A [PM150710]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	2-3 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The U-2 OS [U2OS] cell line was established in 1964 by J. Pontén and E. Saksela from a moderately differentiated sarcoma of the tibia in a 15-year-old female. When co-cultured with WI-38 cells or tested by complement fixation (CF) assay for simian virus 40 (SV40), respiratory syncytial virus (RSV), or adenoviruses, no viruses were detected. U-2 OS cells express insulin-like growth factor I (IGF-I) and II (IGF-II) receptors, as well as osteosarcoma-derived growth factor (ODGF).
Receptor Expression	insulin-like growth factor I (IGF-I);insulin-like growth factor II (IGF II)
Antigen Expression	Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
Gene Expression	osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)
Biosafety Level	BSL-1