U-87 MG-GFP
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Cat. No.: CL-1108
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CL-1108
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | U-87 MG-GFP |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | glioma cells |
| Tissue | Brain |
| Age | Age unspecified |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is prone to cell aggregation. This can be improved by coating culture vessels with Poly-L-Lysine solution or gelatin. It is recommended to prepare Poly-L-Lysine solution (Cat. No.:PB180523). |
| Complete Medium | MEM, with NEAA [PM150410]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 1-2 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The U‑87 MG cell line was established by Pontén J et al. and derived from human malignant glioma. U‑87 MG cells are tumorigenic following subcutaneous inoculation in nude mice. The U‑87 MG‑GFP cell line was constructed from U‑87 MG cells by lentiviral transduction and is a stable cell line capable of long‑term, high‑level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for assays such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 3.0 μg/mL. |
| Tumorigenic | Yes, in nude mice inoculated subcutaneously with 1×10⁷ cells. |
| Biosafety Level | BSL-2 |
MSDS
