U-937

Name: U-937
Brand: Procell
SKU: CL-0239
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0239

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1×10^6Cells/Vial×2Vials

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General information

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	5×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	90% FBS+10% DMSO
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The U-937 cell line was established by Sundström and Nilsson in 1974 from pleural effusion of a patient with histiocytic lymphoma. Studies have shown that U-937 cells can be induced to undergo terminal monocytic differentiation by human mixed lymphocyte culture supernatant, phorbol ester, vitamin D₃, γ-interferon, tumor necrosis factor, and retinoic acid. U-937 cells are negative for immunoglobulin production and EBV expression. The cells express Fas antigen and are sensitive to TNF and anti-Fas antibodies.
Receptor Expression	complement (C3)
Gene Expression	lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)
Biosafety Level	BSL-1