U-937-GFP
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Cat. No.: CL-1091
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CL-1091
$ 720.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | U-937-GFP |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Monocytic |
| Disease | lymphoma cells |
| Tissue | Pleural effusion |
| Age | 37Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 5×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | The U-937 cell line was established by Sundström and Nilsson in 1974 from pleural effusion of a patient with histiocytic lymphoma. Studies have shown that U-937 cells can be induced to undergo terminal monocytic differentiation by human mixed lymphocyte culture supernatant, phorbol ester, vitamin D₃, γ-interferon, tumor necrosis factor, and retinoic acid. U-937 cells are negative for immunoglobulin production and EBV expression. The cells express Fas antigen and are sensitive to TNF and anti-Fas antibodies. The U-937-GFP cell line was constructed from U-937 cells by lentiviral transduction and is a stable cell line capable of long-term, high-level GFP expression. This cell line exhibits low passage, high viability, and good condition, making it suitable for experiments such as flow cytometry and fluorescence imaging. Selection drug concentration: Puro = 2.0 μg/mL. |
| Biosafety Level | BSL-2 |
MSDS
