VK2/E6E7

Name: VK2/E6E7
Brand: Procell
SKU: CL-1024
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-1024

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CL-1024

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	VK2/E6E7
Synonyms	Vk2/E6E7;VK-2/E6E7;VK2 (E6/E7);VK2
Cellosaurus Accession	CVCL_6471
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like
Tissue	Vagina; Mucosa
Age	32Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	Complete culture medium for VK2/E6E7
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:3-1:6
Medium Renewal	2 to 3 times per week
Dissociation Duration	5-7 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	VK2/E6E7 is an epithelial cell line isolated in 1996 from the vaginal mucosa of a 32-year-old female patient who underwent hysterectomy for endometriosis.
Gene Expression	细胞角蛋白8 (CK8)、10 (CK10)、13 (CK13)、18 (CK18)、19(CK19)；巨噬细胞集落刺激因子；转化生长因子β1；白细胞介素8；前列腺素E2；分泌性白细胞蛋白酶抑制剂；聚合免疫球蛋白受体。
Biosafety Level	BSL-1