VMM39
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Cat. No.: CL-0986
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CL-0986
$ 600.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | VMM39 |
| Cellosaurus Accession | CVCL_A739 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | melanoma cells |
| Tissue | Skin; derived from metastatic site: lymph node |
| Age | 71Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. The cell doubling time is approximately 48 hours. Subculture is recommended when cells reach 80% confluence; overgrowth should be avoided. Dark granules increase at high cell density. 4. Numerous dark granules are present in the cell culture background. The culture medium tends to turn yellow, so timely medium exchange and washing are required. 5. Upon recovery, some cells remain suspended and do not adhere, appearing as bright round cells under the microscope. Cells appear to be in fair condition, but when the supernatant is collected and cultured, the cells do not proliferate. The same situation occurs after subculture. Therefore, perform medium exchange 12 hours after recovery or subculture to remove the non-adherent fraction. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | VMM39 was derived from tumor‑involved lymph nodes obtained from patients at the University of Virginia. |
| Antigen Expression | Low GP100, Tyrosinase, Low MAGE-A1, MMP-1 |
| Biosafety Level | BSL-1 |
MSDS
