WERI-Rb-1

Name: WERI-Rb-1
Brand: Procell
SKU: CL-0465
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0465

Publications(7)

CL-0465

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	WERI-Rb-1
Synonyms	WERI-RB-1;WERI-Rb 1;WERI-Rb1;WERI-RB1;WERI Rb-1;WERI;Wills Eye Research Institute-Retinoblastoma-1
Cellosaurus Accession	CVCL_1792
Organism	Human
Growth Properties	Suspension
Morphology	Round (grape)
Tissue	Eye; Retina
Age	1Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1×10⁵-2×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background

The WERI-Rb-1 cell line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery. WERI-Rb-1 cells can survive in Difco Bacto-Agar but do not form colonies. Scanning electron microscopy reveals variations in the number and frequency of surface adornments such as blebs, lamellipodia, and microvilli. WERI-Rb-1 cells are used in studies on cell differentiation, animal models for tumor therapy, and biochemical evaluation.

Biosafety Level

BSL-1